Journal: Medical Oncology (Northwood, London, England)

Article Title: Ashwagandha ( Withania somnifera ) targets liver cancer stem cells via inhibiting Hedgehog signaling pathway in hepatocellular carcinoma

doi: 10.1007/s12032-025-03215-0

Figure Lengend Snippet: Flow cytometry analysis of CD90 expression in HepG2 cells treated with SOR and/or ASH-AE for 48 h. *: Statistically significant difference from control group at p ≤ 0.05, #: Statistically significant difference from SOR group at p ≤ 0.05

Article Snippet: After 24 h, cells were treated with IC50 of SOR and/or ASH-AE in triplicates and incubated in the same conditions for 48 h. After that, cells were collected and 1 × 10 6 cells were resuspended in 100 μl PBS and then incubated with PE/Elab Fluor ® 594 Anti-Human CD90 Antibody (1:50 dilution) (Elabscience, USA) for 30 min at room temperature.

Techniques: Flow Cytometry, Expressing, Control

Journal: Biology

Article Title: Total Flavonoids of Rhizoma drynariae Enhance Bone Marrow Mesenchymal Stem Cell-Mediated Tendon–Bone Healing by Promoting Tissue Regeneration, Angiogenesis, and Modulation of Cytokine Expression

doi: 10.3390/biology14111593

Figure Lengend Snippet: Morphological observation and flow cytometric characterization of rat BMSCs. ( A ) Primary (P0) BMSCs showed granular colony-like clusters (100×); ( B ) Passage 1 (P1) BMSCs exhibited short spindle-shaped adherent growth (100×); ( C ) Passage 3 (P3) BMSCs displayed elongated spindle-shaped morphology with adherent growth (100×); ( D ) Flow cytometric analysis of CD29 expression; ( E ) CD90 expression; ( F ) CD34 expression.

Article Snippet: The key reagents and instruments used in this study included the CCK-8 assay kit (Beijing Dingguosheng Biotechnology Co., Ltd., Beijing, China), osteogenic and chondrogenic induction kits for rat BMSCs, and angiogenesis induction medium for HUVECs (Saiye Biotechnology Co., Ltd., Guangzhou, China), Matrigel matrix gel (Corning Life Sciences, Wujiang, China), CD29-APC and CD90-PE-CY7 antibodies (Elabscience Biotechnology Co., Ltd., Wuhan, China), CD34-PE antibody (Bioss Biotechnology Co., Ltd., Beijing, China), Qianggu capsules (Beijing Qihuang Pharmaceutical Co., Ltd., Beijing, China; Batch/Lot No.: Z20030007), hematoxylin–eosin (H & E) staining solution, Masson’s trichrome staining kit, Safranin O–Fast Green staining kit (BioS Biotech Co., Ltd., Hubei, China), ELISA kits (Kedi Yunxiang Biotech Co., Ltd., Nanjing, China), cell migration analysis plug-in (Shenzhen Kuyuan Biotech Co., Ltd., Shenzhen, China), and a microcomputer-controlled universal testing machine (Bairo Testing Equipment Co., Ltd., Shanghai, China).

Techniques: Expressing

Journal: iScience

Article Title: Identification of optimal conditions for human placental explant culture and extracellular vesicle release

doi: 10.1016/j.isci.2023.108046

Figure Lengend Snippet:

Article Snippet: Human anti-CD90 PE , Cellarcus Biosciences , Cat# CBS83-PE-100T.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Proliferation Assay, LDH Cytotoxicity Assay, Software